Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human VEGF-D protein
Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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