Lyophilized from 0.22 µm filtered solution in 0.085% TFA in 30% ACN with trehalose as protectant
Quelle:
Human
Reinheit:
95%
Formulierung:
Powder
Target-Kategorie:
TGF-beta 1
Anwendungsbeschreibung:
Application Notes: This protein carries no tag. The protein has a calculated MW of 12.8 kDa (monomer). The protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE)
SDS-PAGE analysis of Human TGF-beta 1 protein
Immobilized Human TGFB1, premium grade (Cat. No. orb257996) at 0.2 µg/mL (100 µL/well) can bind Human TGFBR2, Fc Tag (Cat. No. orb257866) with a linear range of 0.2-2.5 ng/mL (QC tested).
Human TGF-beta 1 Protein (Cat. No. orb257996) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Human TGF-beta 1 Protein (Cat. No. orb257996) immobilized on CM5 Chip can bind Human TGF-beta RI Protein, Fc Tag (Cat. No. orb1786177) with an affinity constant of 99.2 nM as determined in a SPR assay (Biacore 8K)
Human TGF-beta 1 Protein (Cat. No. orb257996) inhibits the Human IL-4, premium grade (Cat. No. orb257598) dependent proliferation the TF-1 cells. The specific activity of Human TGF-Beta 1 Protein, Tag Free is > 8.0010 6 IU/mg, which is calibrated against transforming growth factor beta1 (NIBSC code: 89/514)
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