A synthetic peptide corresponding to a sequence in the middle region of human Annexin A3, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.
Konjugation:
Unconjugated
Alternative Synonym:
Annexin A3, 35-alpha calcimedin, Annexin III, Annexin-3, Inositol 1, 2-cyclic phosphate 2-phosphohydrolase, Lipocortin III, Placental anticoagulant protein III, PAP-III, ANXA3, ANX3
Annexin A3/ANXA3 Antibody
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Application Notes: Immunohistochemistry (Frozen Section), 0.5-1µg/mlImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/mlWestern blot, 0.1-0.5µg/mlImmunocytochemistry , 0.5-1µg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in frozen section of IHC(F): Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody.Annexin A3 was detected in pa
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in frozen section of IHC(F): Mouse Cardiac Muscle Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in frozen section of IHC(F): Rat Cardiac Muscle Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in paraffin-embedded section of Mouse Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Annexin A3 using anti-Annexin A3 antibody. Annexin A3 was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Annexin A3 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of Annexin A3 using anti-Annexin A3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking ge
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
