Chicken Prostaglandin E2 (PGE2) ELISA Kit

Artikelnummer: BYT-ORB2667419
Artikelname: Chicken Prostaglandin E2 (PGE2) ELISA Kit
Artikelnummer: BYT-ORB2667419
Hersteller Artikelnummer: orb2667419
Alternativnummer: BYT-ORB2667419-48,BYT-ORB2667419-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Gallus
Alternative Synonym: PG-E2
Chicken Prostaglandin E2 (PGE2) ELISA Kit
Detektionsbereich: 31.25-2000 pg/mL
Sensitivitaet: 10.3 pg/mL
Proben: serum, plasma and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken PGE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken PGE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chicken PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve