Human p-cresol ELISA Kit

Artikelnummer: BYT-ORB2667435
Artikelname: Human p-cresol ELISA Kit
Artikelnummer: BYT-ORB2667435
Hersteller Artikelnummer: orb2667435
Alternativnummer: BYT-ORB2667435-48,BYT-ORB2667435-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: 4-Hydroxytoluene, 4-Methylphenol, p-Cresol 98+ %, PARA-CRESOL, 4-cresol, para cresol
Human p-cresol ELISA Kit
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 4.8 pg/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve