Simian RBP4(Retinol Binding Protein 4, Plasma) ELISA Kit
Artikelnummer:
BYT-ORB3126874
Hersteller Artikelnummer:
orb3126874
Alternativnummer:
BYT-ORB3126874-48, BYT-ORB3126874-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Spezies Reaktivität:
Primate
Alternative Synonym:
PRBP, RBP, Plasma retinol-binding protein
Simian RBP4(Retinol Binding Protein 4, Plasma) ELISA Kit
Detektionsbereich:
0.78-50 µg/mL
Sensitivitaet:
0.35 µg/mL
Proben:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian RBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian RBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian RBP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian RBP4 in the samples is then determined by comparing the OD of the samples to the standard curve
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