Simian HMGB-1(High Mobility Group Protein B1) ELISA Kit

Artikelnummer: BYT-ORB3126989
Artikelname: Simian HMGB-1(High Mobility Group Protein B1) ELISA Kit
Artikelnummer: BYT-ORB3126989
Hersteller Artikelnummer: orb3126989
Alternativnummer: BYT-ORB3126989-48, BYT-ORB3126989-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Primate
Alternative Synonym: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein, HMG1, High Mobility Group Protein 1
Simian HMGB-1(High Mobility Group Protein B1) ELISA Kit
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.064 ng/mL
Proben: serum, plasma and other biological fluids
Anwendungsbeschreibung: Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve