EliKine Human IFN-alpha ELISA Kit

Produktübersicht

• Artikelname: EliKine Human IFN-alpha ELISA Kit
• Artikelnummer: BYT-ORB3145525
• Hersteller Artikelnummer: orb3145525
• Alternativnummer: BYT-ORB3145525-48, BYT-ORB3145525-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: IFNA1, IFL, IFN, IFNA, IFNA13, IFN-ALPHA, IFN-alphaD

Produktbeschreibung

EliKine Human IFN-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Human IFN-alpha.

Produkteigenschaften

• Detektionsbereich: 46.9 pg/mL - 3000 pg/mL
• Sensitivitaet: 46.9 pg/mL
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: EliKine Human IFN-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-alpha in samples. An antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured