EliKine Mouse IL-2 ELISA Kit

Artikelnummer: BYT-ORB3145555
Artikelname: EliKine Mouse IL-2 ELISA Kit
Artikelnummer: BYT-ORB3145555
Hersteller Artikelnummer: orb3145555
Alternativnummer: BYT-ORB3145555-48, BYT-ORB3145555-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: IL 2, IL2, IL-2, TCGF, lymphokine, T cell growth factor, aldesleukin, interleukin-2, involved in regulation of T-cell clonal expansion
EliKine Mouse IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-2.
Detektionsbereich: 3.13 pg/mL- 200 pg/mL
Sensitivitaet: 1.6 pg/mL
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Mouse IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-2 in samples. An antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-2 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured