EliKine Mouse IL-5 ELISA Kit

Artikelnummer: BYT-ORB3145557
Artikelname: EliKine Mouse IL-5 ELISA Kit
Artikelnummer: BYT-ORB3145557
Hersteller Artikelnummer: orb3145557
Alternativnummer: BYT-ORB3145557-48, BYT-ORB3145557-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: IL 5, IL5, IL-5, IL-5T-cell replacing factor, interleukin-5, TRF, TRFB cell differentiation factor I
EliKine Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-5.
Detektionsbereich: 7.8 pg/mL-500 pg/mL
Sensitivitaet: 4 pg/mL
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured