EliKine Mouse IL-10 ELISA Kit

Artikelnummer: BYT-ORB3145559
Artikelname: EliKine Mouse IL-10 ELISA Kit
Artikelnummer: BYT-ORB3145559
Hersteller Artikelnummer: orb3145559
Alternativnummer: BYT-ORB3145559-48, BYT-ORB3145559-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: IL 10, IL10, CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
EliKine Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-10.
Detektionsbereich: 93.75 pg/mL- 6000 pg/mL
Sensitivitaet: 50 pg/mL
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured