EliKine Rat IL-1beta ELISA Kit

Produktübersicht

• Artikelname: EliKine Rat IL-1beta ELISA Kit
• Artikelnummer: BYT-ORB3145568
• Hersteller Artikelnummer: orb3145568
• Alternativnummer: BYT-ORB3145568-48, BYT-ORB3145568-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Rat
• Alternative Synonym: IL1B, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta

Produktbeschreibung

EliKine Rat IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-1beta.

Produkteigenschaften

• Detektionsbereich: 62.5 pg/ml-4000 pg/ml
• Sensitivitaet: 32 pg/mL
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: EliKine Rat IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1beta in samples. An antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured