EliKine Rat IL-6 ELISA Kit

Artikelnummer: BYT-ORB3145571
Artikelname: EliKine Rat IL-6 ELISA Kit
Artikelnummer: BYT-ORB3145571
Hersteller Artikelnummer: orb3145571
Alternativnummer: BYT-ORB3145571-48, BYT-ORB3145571-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rat
Alternative Synonym: IL 6, IL6, BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2, B-cell differentiation factor, CTL differentiation factor, OTTHUMP00000158544, hybridoma growth factor, interleukin 6, interleukin BSF-2
EliKine Rat IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-6.
Detektionsbereich: 250pg/mL-16000 pg/mL
Sensitivitaet: 100 pg/mL
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: EliKine Rat IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-6 in samples. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured