Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit

Artikelnummer: BYT-ORB3145808
Artikelname: Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit
Artikelnummer: BYT-ORB3145808
Hersteller Artikelnummer: orb3145808
Alternativnummer: BYT-ORB3145808-48,BYT-ORB3145808-96,BYT-ORB3145808-5,BYT-ORB3145808-50
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: USP14, TGT, deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kD subunit, ubiquitin carboxyl-terminal hydrolase 14, ubiquitin specific protease 14, ubiquitin specific protease 14 (tRNA-guanine tran
This Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit employs a two-site sandwich ELISA to quantitate USP14.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) ELISA Kit employs a two-site sandwich ELISA to quantitate USP14 in samples. An antibody specific for USP14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP14 bound in the initial step. The color development is stopped and the intensity of the color is measured