Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit

Artikelnummer: BYT-ORB3145829
Artikelname: Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit
Artikelnummer: BYT-ORB3145829
Hersteller Artikelnummer: orb3145829
Alternativnummer: BYT-ORB3145829-48,BYT-ORB3145829-96,BYT-ORB3145829-5,BYT-ORB3145829-50
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: UBE2C, RP3-447F3.1, UBCH10, dJ447F3.2, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, ubiquitin carrier protein E2-C, ubiquitin-protein ligase C
This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C in samples. An antibody specific for UBE2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2C bound in the initial step. The color development is stopped and the intensity of the color is measured