Human Protein kinase C zeta type (PRKCZ) ELISA Kit

Artikelnummer: BYT-ORB3146869
Artikelname: Human Protein kinase C zeta type (PRKCZ) ELISA Kit
Artikelnummer: BYT-ORB3146869
Hersteller Artikelnummer: orb3146869
Alternativnummer: BYT-ORB3146869-48, BYT-ORB3146869-5, BYT-ORB3146869-50, BYT-ORB3146869-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PRKCZ, PKC-ZETA, PKC2,
This Human Protein kinase C zeta type (PRKCZ) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCZ.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Protein kinase C zeta type (PRKCZ) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCZ in samples. An antibody specific for PRKCZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCZ bound in the initial step. The color development is stopped and the intensity of the color is measured