Human Prickle-like protein 3 (PRICKLE3) ELISA Kit

Artikelnummer: BYT-ORB3146884
Artikelname: Human Prickle-like protein 3 (PRICKLE3) ELISA Kit
Artikelnummer: BYT-ORB3146884
Hersteller Artikelnummer: orb3146884
Alternativnummer: BYT-ORB3146884-48, BYT-ORB3146884-5, BYT-ORB3146884-50, BYT-ORB3146884-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PRICKLE3, LMO6, LIM domain only 6
This Human Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3 in samples. An antibody specific for PRICKLE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE3 bound in the initial step. The color development is stopped and the intensity of the color is measured