Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit

Artikelnummer: BYT-ORB3146935
Artikelname: Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit
Artikelnummer: BYT-ORB3146935
Hersteller Artikelnummer: orb3146935
Alternativnummer: BYT-ORB3146935-48, BYT-ORB3146935-5, BYT-ORB3146935-50, BYT-ORB3146935-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PIGL, N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, N-acetylglucosaminylphosphatidylinositol deacetylase, phosphatidylinositol glycan, class L
This Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGL.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGL in samples. An antibody specific for PIGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGL bound in the initial step. The color development is stopped and the intensity of the color is measured