Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit

Artikelnummer: BYT-ORB3146961
Artikelname: Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit
Artikelnummer: BYT-ORB3146961
Hersteller Artikelnummer: orb3146961
Alternativnummer: BYT-ORB3146961-48, BYT-ORB3146961-5, BYT-ORB3146961-50, BYT-ORB3146961-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PGAM1, RP11-452K12.8, PGAM-B, PGAMA, BPG-dependent PGAM 1, phosphoglycerate mutase 1, phosphoglycerate mutase A, nonmuscle form
This Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGAM1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGAM1 in samples. An antibody specific for PGAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured