Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit

Artikelnummer: BYT-ORB3146966
Artikelname: Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit
Artikelnummer: BYT-ORB3146966
Hersteller Artikelnummer: orb3146966
Alternativnummer: BYT-ORB3146966-48, BYT-ORB3146966-5, BYT-ORB3146966-50, BYT-ORB3146966-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PFKM, GSD7, MGC8699, PFK-1, PFK1, PFKA, PFKX, 6-phosphofructo-1-kinase, phosphofructo-1-kinase isozyme A, phosphofructokinase, polypeptide X, phosphohexokinase
This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM in samples. An antibody specific for PFKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKM bound in the initial step. The color development is stopped and the intensity of the color is measured