Human Platelet endothelial cell adhesion molecule (PECAM1) ELISA Kit

Artikelnummer: BYT-ORB3146978
Artikelname: Human Platelet endothelial cell adhesion molecule (PECAM1) ELISA Kit
Artikelnummer: BYT-ORB3146978
Hersteller Artikelnummer: orb3146978
Alternativnummer: BYT-ORB3146978-48, BYT-ORB3146978-5, BYT-ORB3146978-50, BYT-ORB3146978-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PECAM1, CD31, FLJ58394, PECAM-1, CD31 antigen, CD31/EndoCAM, PECAM-1, CD31/EndoCAM, adhesion molecule
This Human Platelet endothelial cell adhesion molecule (PECAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PECAM1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: Application Notes: This Human Platelet endothelial cell adhesion molecule (PECAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PECAM1 in samples. An antibody specific for PECAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPECAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PECAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PECAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured