Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit

Produktübersicht

• Artikelname: Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit
• Artikelnummer: BYT-ORB3146988
• Hersteller Artikelnummer: orb3146988
• Alternativnummer: BYT-ORB3146988-48, BYT-ORB3146988-5, BYT-ORB3146988-50, BYT-ORB3146988-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: PDHB, DKFZp564K0164, PHE1B, Pyruvate dehydrogenase, E1 beta polypeptide

Produktbeschreibung

This Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB.

Produkteigenschaften

• Detektionsbereich: Please inquire
• Sensitivitaet: Please inquire
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: This Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB in samples. An antibody specific for PDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHB bound in the initial step. The color development is stopped and the intensity of the color is measured