Human Poly ADP ribose polymerase (PARP) ELISA Kit

Produktübersicht

• Artikelname: Human Poly ADP ribose polymerase (PARP) ELISA Kit
• Artikelnummer: BYT-ORB3147025
• Hersteller Artikelnummer: orb3147025
• Alternativnummer: BYT-ORB3147025-48, BYT-ORB3147025-5, BYT-ORB3147025-50, BYT-ORB3147025-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: PARP1, RP11-125A15.2, ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), poly (ADP-ribose) polymerase family, member 1, po

Produktbeschreibung

This Human Poly ADP ribose polymerase (PARP) ELISA Kit employs a two-site sandwich ELISA to quantitate PARP1.

Produkteigenschaften

• Detektionsbereich: Please inquire
• Sensitivitaet: Please inquire
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: This Human Poly ADP ribose polymerase (PARP) ELISA Kit employs a two-site sandwich ELISA to quantitate PARP1 in samples. An antibody specific for PARP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP1 bound in the initial step. The color development is stopped and the intensity of the color is measured