Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit

Produktübersicht

• Artikelname: Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit
• Artikelnummer: BYT-ORB3147033
• Hersteller Artikelnummer: orb3147033
• Alternativnummer: BYT-ORB3147033-48, BYT-ORB3147033-5, BYT-ORB3147033-50, BYT-ORB3147033-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: PAM, PAL, PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylglycine 2-hydroxylase, peptidylglycine a

Produktbeschreibung

This Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit employs a two-site sandwich ELISA to quantitate PAM.

Produkteigenschaften

• Detektionsbereich: Please inquire
• Sensitivitaet: Please inquire
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: This Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit employs a two-site sandwich ELISA to quantitate PAM in samples. An antibody specific for PAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAM bound in the initial step. The color development is stopped and the intensity of the color is measured