Application Notes: This Mouse Speckle-type POZ protein (SPOP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOP in samples. An antibody specific for SPOP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOP bound in the initial step. The color development is stopped and the intensity of the color is measured
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