RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG2a confirmed by Dot Blot.
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
ELISA: User Optimized, FLISA: User Optimized, FC: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Biological Origin: Rat. Application Notes: RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user
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