synthetic FLAG peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG
DYKDDDDK (FLAG) peptide
Konzentration:
1 mg/mL
Puffer:
None
Reinheit:
Greater than 90% specific peptide by HPLC and MS.
Formulierung:
Lyophilized
Application Verdünnung:
IP: User Optimized - see PROTOCOL, WB: User Optimized as blocking peptide in PCA
Anwendungsbeschreibung:
Application Notes: The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 µg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use
SDS-PAGE of Anti-DYKDDDDK (FLAG tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAG tag) recombinant protein (arrowhead). Load: (6 µl per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAG tag) Affinity Gel. Lane 1: crude lysate containing over-expressed DYKDDDK-tagged recombinant protein. Lane 2: unbound flow-through of consisting of endogenous E.coli proteins. Lane 3: enriched recombinant protein. Load: 5 µg protein, 15 µl flow through. Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction 1. Lane 5: eluted fraction 2. Lane 6: eluted fraction 3. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15 µl per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.
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