Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
Application Notes: Anti-Acetyl Cholinesterase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,200 to 1:5,000 of the reconstitution concentration is suggested for this product
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