This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(c). No reaction was observed against anti-Goat IgG F(ab)2 or anti-Papain.
Formulierung:
Liquid (sterile filtered)
Anwendungsbeschreibung:
Biological Origin: Goat. Application Notes: Goat IgG F(c) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments
SDS PAGE Results of Goat IgG F(c) Fragment. Lane 1: Goat IgG F(c) Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Goat IgG F(c) Non-Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab), 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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