This product was prepared from normal serum by delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human Transferrin and anti-Human Serum.
Formulierung:
Lyophilized
Application Verdünnung:
IF: 1:500-1:2500
Anwendungsbeschreibung:
Biological Origin: Human. Application Notes: Human transferrin fluorescein conjugation has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms
Dot Blot of Fluorescein conjugated Human Transferrin. Antigen: Human Transferrin Fluorescein. Load: 3-fold serial dilution starting at 200 ng. Block: None.
Dot Blot of Fluorescein conjugated Human Transferrin. Antigen: Human Transferrin Fluorescein. Load: 3-fold serial dilution starting at 200 ng. Block: orb348637 for 30 min at RT.
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