Human IgM whole molecule Biotin conjugated, human myeloma IgM whole molecule biotin conjugation
Human IgM (myeloma) Biotin Antibody
Konzentration:
1.0 mg/mL
Isotyp:
IgM
Puffer:
0.01% (w/v) Sodium Azide
Quelle:
Human
Reinheit:
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation, ion exchange chromatography followed by tandem molecular sieve chromatography and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
Formulierung:
Lyophilized
Anwendungsbeschreibung:
Biological Origin: Human. Application Notes: Human IgM (myeloma) Biotin conjugated whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting
Characterization of a Star635P-conjugated polyclonal antihuman IgM Fab. a Epifluorescence images of wild type and IgM-negative (IgMneg) Ramos B cells stained with a monovalent Star635P-conjugated Fab against human IgM (Fab-Star635P). Fluorescent images were acquired under the same conditions and equally scaled to allow a direct comparison. Transmission light inset in the image on the right-hand side shows the presence of IgMneg cells. Scale bars represent 10 µm. b Ca2 + mobilization analysis of wild type Ramos B cells stimulated (indicated by an arrow) with either polyclonal anti-IgM F(ab)2 (blue curve) or the Star635P-conjugated monovalent anti-IgM Fab (red curve). c Wild type Ramos B cells were stained with increasing concentrations of Fab-Star635P to establish the optimal concentration needed for saturated labeling of mIgM-BCRs on the cell surface. Dilutions ranged from 1:10 (0.1%) to 1:1000 (0.001%) from our stock of fluorescently-labeled Fab. After background correction regions of interest containing whole cells were manually selected and the average intensity of whole cells was calculated. Values represent the average of cellular 6 fluorescence intensities from > 50 cells per concentration point. Error bars represent the standard error of the mean. The arrow indicates the final concentration that was used in the experiments shown. d STED images of single Fab-Star635P and Fab-Star635P mixed with a monomeric Ig µ heavy chain seeded on glass coverslips (scale bar 1 µm). The bar graph shows the mean fluorescence intensity of single spots and error bars represent the standard error of the mean from eight independent experiments. e, f Confocal images of plasma membrane sheets stained with R18 (pseudocolored in green) and STED images showing the Fab-Star635P fluorescence signal derived from either untreated Ramos B cells (e) or cells that were BCR-activated with a monoclonal anti-IgM antibody (f). Membrane sheets were analyzed as described for Figure 1. Example histograms showing the fluorescence intensity distributions of Fab-Star635Pconjugated on coverslips (black curves, control) and mIgM-spots present in membrane sheets (red curves, mIgM-BCRs).
SARS-CoV-2 N protein amino acid coverage on SARS-CoV-2 microarray. (A) Final N protein constructs fabricated on the SARS-CoV-2 microarray, including the full-length protein, core domain (amino acids 44-362), N-terminal domain (NTD) (amino acids 43-179), C-terminal domain (CTD) (amino acids 246-363), peptide 6 (amino acids 395-412), peptide 8 (amino acids 211-228) and peptide 16 (amino acids 367-389). (B) Final microarray layout: 24-plex array with 16 probes (summarized in the Table insert) printed in triplicate. Control antigens used in the microarray included 50 µg/ml of biotinylated human immunoglobulins G, A, and M (hIgG, hIgA, and hIgM, respectively) and 132 µg/ml of biotinylated anti-human immunoglobulin G (anti-hIgG) as well as in house derivatized NHS-ester-Cy3 biotinylated BSA (Cy3-BSA) at 40 µg/ml.
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