Rabbit IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecule Biotin conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG and anti-Rabbit Serum.
Formulierung:
Lyophilized
Application Verdünnung:
ELISA: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Biological Origin: Rabbit. Application Notes: Rabbit IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments. A streptavidin-conjugated reporter (ie. Streptavidin Peroxidase Conjugated) can be used for detection
Identification and characterization of lung fibrocytes in IPF lungs. b Representative gating strategy for lung fibrocytes (CD45 + CD15-CD3-CD19-CD56-CXCR4+Col-1+-cells) in explanted IPF lungs (n = 3 bottom) and control lungs (HC, top) (n = 4). Single cell suspensions were thawed and alive viable cells were further analyzed. Lung fibrocytes are present in the CD45+ cell population. Contaminating and unwanted CD15+ neutrophils, CD3+ T cells, CD19+ B cells and CD56+ NK-cells were sequentially excluded. Isotype control for Col-1 was used to set the gate for Col-1 + cells. To control for non-specific labeling Rabbit IgG-Biotin conjugated was used.
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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