HSP90 alpha/beta Antibody (Biotin), IgG2a, Clone: [Hyb-K41220A], Mouse, Monoclonal

Produktübersicht

• Artikelname: HSP90 alpha/beta Antibody (Biotin), IgG2a, Clone: [Hyb-K41220A], Mouse, Monoclonal
• Artikelnummer: BYT-ORB396201
• Hersteller Artikelnummer: orb396201
• Alternativnummer: BYT-ORB396201-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat, Saccharomyces, Yeast
• Immunogen: Recombinant human HSP90alpha, Specificity mapped to amino acids 291-304
• Konjugation: Biotin
• Alternative Synonym: HSP90AA1, HSP90AB1, HSP90-alpha, HSP90-beta, HSP90A, HSP90B, HSP89A, HSP86, HSP84, HSP90Alpha, HSPCA, HSPC1, HSPC2, HSPCB, HSPCAL3, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Heat shock 90 kDa protein 1 alpha isoform, Heat shock 84 kDa protein, D6S182, FLJ26984

Produktbeschreibung

Mouse monoclonal antibody against HSP90 alpha/beta conjugated to Biotin.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [Hyb-K41220A]
• Molekulargewicht: 90kDa
• Isotyp: IgG2a
• NCBI: 031381
• UniProt: P08238
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target-Kategorie: HSP90 alpha/beta

Anwendungsbeschreibung

• Application Verdünnung: WB (1:1000), IHC (1:100), ICC/IF (1:100)
• Anwendungsbeschreibung: Application Notes: 1 µg/ml was sufficient for detection of HSP90alphabeta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary

Bilder

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.