O-GalNAC Antibody (Biotin), IgM, Clone: [9B9], Mouse, Monoclonal

Produktübersicht

• Artikelname: O-GalNAC Antibody (Biotin), IgM, Clone: [9B9], Mouse, Monoclonal
• Artikelnummer: BYT-ORB396453
• Hersteller Artikelnummer: orb396453
• Alternativnummer: BYT-ORB396453-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC, WB
• Spezies Reaktivität: All
• Immunogen: Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Konjugation: Biotin
• Alternative Synonym: i-O-Linked N-Acetylglucosamine, O-Linked N-Acetylglucosamine, O-GlcNAc, Beta-O-Linked N-Acetylglucosamine, N-Acetylglucosamine, GlcNac, 2-(Acetylamino)-2-deoxy-D-glucose, N-acetyl-D-glucosamine, NAG, i-D-(Acetylamino)-2-deoxy-glucopyranose, beta-N-Acetylglucosamine, N-Acetylglucosamine-modified protein, i-N-Acetylglucosamine

Produktbeschreibung

Mouse monoclonal antibody against O-GalNAC conjugated to Biotin.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [9B9]
• Isotyp: IgM
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target-Kategorie: O-GalNAC

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000, IF/ICC: 1:50, ELISA: 1:1000, IHC-P: 1:25
• Anwendungsbeschreibung: Application Notes: A 1:10,000 dilution of SMC-503 was sufficient for detection of O-GalNAc in 2 µg of O-GalNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain, DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633, DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.

Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.