NR1B1, Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1
The RAR alpha Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human RAR alpha .Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Application Verdünnung:
WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
Western blot analysis of RAR alpha expression in MCF7 (A), HepG2 (B), mouse lung (C), mouse liver (D) whole cell lysates. (Predicted band size: 39, 50 kD, Observed band size: 60 kD)
Immunofluorescent analysis of RAR alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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