The ILK Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human ILK .Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Application Verdünnung:
WB: 1:500-2000
Western blot analysis of ILK expression in Hela (A), A549 (B), NIH3T3 (C), mouse lung (D) whole cell lysates. (Predicted band size: 36, 44, 51 kD, Observed band size: 51 kD)
Immunohistochemical analysis of ILK staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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