HSP70 (Acetyl Lys77) Antibody (Biotin), Rabbit, Polyclonal

Produktübersicht

• Artikelname: HSP70 (Acetyl Lys77) Antibody (Biotin), Rabbit, Polyclonal
• Artikelnummer: BYT-ORB414154
• Hersteller Artikelnummer: orb414154
• Alternativnummer: BYT-ORB414154-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Konjugation: Biotin
• Alternative Synonym: HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, HSP70 Acetylated lysine 77, HSP70 Acetyl Lys77

Produktbeschreibung

Rabbit polyclonal antibody against HSP70 Acetyl Lys77 conjugated to Biotin

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5mg/mL
• Molekulargewicht: 70 kDa
• NCBI: 005336
• UniProt: P0DMV8
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target-Kategorie: HSP70 (Acetyl Lys77)

Anwendungsbeschreibung

• Application Verdünnung: WB (1:1000), ELISA (1:1000), ICC/IF (1:60), IHC (1:50)
• Anwendungsbeschreibung: Application Notes: A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 µM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain, DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 Acetyl Lys77 protein using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.