Human GM CSF Protein

Produktübersicht

• Artikelname: Human GM CSF Protein
• Artikelnummer: BYT-ORB425776
• Hersteller Artikelnummer: orb425776
• Alternativnummer: BYT-ORB425776-1, BYT-ORB425776-20, BYT-ORB425776-5
• Hersteller: Biorbyt
• Kategorie: Proteine/Peptide
• Alternative Synonym: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.

Produktbeschreibung

Recombinant of human GM CSF protein

Produkteigenschaften

• Puffer: GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.40.1.
• Reinheit: Greater than 98.0% as determined by:1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
• Formulierung: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequenz: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically

Anwendungsbeschreibung

• Anwendungsbeschreibung: Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg. Application Notes: Protein content: GM-CSF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard