Histone H3 (di methyl K79) Mouse Monoclonal Antibody, Clone: [2A7], Unconjugated

Produktübersicht

• Artikelname: Histone H3 (di methyl K79) Mouse Monoclonal Antibody, Clone: [2A7], Unconjugated
• Artikelnummer: BYT-ORB499569
• Hersteller Artikelnummer: orb499569
• Alternativnummer: BYT-ORB499569-50,BYT-ORB499569-100,BYT-ORB499569-200
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of di methyl K79 DF(Di methyl K)TD
• Konjugation: Unconjugated
• Alternative Synonym: H3C1 | Histone H3 (me2-K79), me2-Histone H3, di-methyl-Histone H3, H3K79me2, me2-H3, H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3/b, H3C1, H3FB, HIST1H3D, H3.1, H3/c, H3FC, HIST1H3C, H3/d, H3FD, HIST1H3E, H3.f, H3/f, H3FF, HIST1H3I, H3/h, H3FH, HIST1H3G, H3/j, H3FJ, HIST1H3J, H3/k, H3F1K, H3FK, HIST1H3H, H3/l, H3FL, HIST1H3B, H3/i, H3FI, HIST1H3F, H3.2-221, H3c13, H3c14, H3c15, Hist1, H3-143, H3-b, H3-53, H3-f, H3-291, H3-H, H3.1-221, H3 histone, Hist1h2ail, Hist1h2ail1, Hist2h3c, Hist3h3, H31_HUMAN, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FC HIST1H3C, H33_HUMAN, H3-3A, H3-3B, H3.3A, H3F3, H3F3A, H3.3B, H3F3B, H31T_HUMAN, H3-4, H3/t, H3t, H3/g, Histone H3.4, H3FT, H3C_HUMAN, H3-5, Histone H3.5, H3F3C, H32_HUMAN, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, HIST2H3A, H3F2, H3FM, HIST2H3D, H31_MOUSE, H3a, H3g, H3.1-291, H3h, H3.1-I, H3i, H32_MOUSE, H3-clustered histone 2, H3-clustered histone 3, H3-clustered histone 4, H3-clustered histone 6, H3-clustered histone 7, H3.2, H3b, H3f, H3.2-616, Hist2h3b, H3.2-615, Hist2h3c1, Hist2h3ca1, H3.2-614, Hist2h3c2, Hist2h3ca2, H31_RAT,

Produktbeschreibung

Histone H3 (di methyl K79) Mouse Monoclonal Antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1mg/ml
• Klon-Bezeichnung: [2A7]
• Molekulargewicht: 15 kDa
• UniProt: P68431
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: H3C1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:1000-5000, IHC-P=1:500-1000, IHC-F=1:500-1000, IF=1:500-1000

Bilder

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3) Monoclonal Antibody, Unconjugated (orb499569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-Histone H3 (di methyl K79) (orb499569) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 19 kD.

IHC-P analysis of Mouse colon using Histone H3 di methyl K79 Monoclonal Antibody at 1:2000 dilution.