VEGF-C Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: VEGF-C Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB499577
• Hersteller Artikelnummer: orb499577
• Alternativnummer: BYT-ORB499577-50,BYT-ORB499577-100,BYT-ORB499577-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human VEGF-C (112-170/419aa)
• Konjugation: Unconjugated
• Alternative Synonym: Flt4-L, LMPH1D, LMPHM4, VRP, VEGF-C, VEGFC_HUMAN, VEGFC, Flt4 ligand (Flt4-L), Vascular endothelial growth factor-related protein (VRP), VEGFC_MOUSE, VEGFC_RAT,

Produktbeschreibung

VEGF-C Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 13/46 kDa
• UniProt: P49767
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: VEGFC

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (orb499577) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (orb499577) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (orb499577) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (orb499577) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: HUVEC (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-VEGF-C (orb499577) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 45 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-VEGF-C (orb499577) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 13 kD.

Western blot:Spleen (Mouse),Primary:Anti-VEGF-C at 1:300 dilution.