JNK1 + JNK3 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: JNK1 + JNK3 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB499914
• Hersteller Artikelnummer: orb499914
• Alternativnummer: BYT-ORB499914-50,BYT-ORB499914-100,BYT-ORB499914-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human JNK1 + JNK3 (211-300/427aa)
• Konjugation: Unconjugated
• Alternative Synonym: JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, MK08_HUMAN, MAPK8, MAP kinase 8, MAPK 8, Stress-activated protein kinase 1c (SAPK1c), Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, 2.7.11.24, MK08_MOUSE, MK08_RAT, SAPK gamma, p54 gamma,

Produktbeschreibung

JNK1 + JNK3 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 42 kDa
• UniProt: P45983
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: MAPK8

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Bilder

Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-JNK1 + JNK3 antibody (orb499914), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-JNK1 + JNK3 antibody (orb499914), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (JNK1 + JNK3) polyclonal Antibody, Unconjugated (orb499914) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1 + JNK3) Polyclonal Antibody, Unconjugated (orb499914) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1 + JNK3) Polyclonal Antibody, Unconjugated (orb499914) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1 + JNK3) Polyclonal Antibody, Unconjugated (orb499914) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-JNK1 + JNK3 (orb499914) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46/54 kD, Observed band size: 46 kD.