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Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (orb5478) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of Rat liver using HSD3B1 Polyclonal Antibody at 1:400 dilution. |
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IHC-P analysis of mouse placenta using HSD3B1 Polyclonal Antibody at 1:200 dilution. |