Phospho-IKK beta (Tyr199) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-IKK beta (Tyr199) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB5519
• Hersteller Artikelnummer: orb5519
• Alternativnummer: BYT-ORB5519-100,BYT-ORB5519-200,BYT-ORB5519-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKK beta around the phosphorylation site of Tyr199 QK(p-Y)TV
• Konjugation: Unconjugated
• Alternative Synonym: IKK beta(phospho Y199), IKK beta(phospho Tyr199), p-IKK beta(Tyr199), IKKI kappa B kinase 2, IKKbeta, IKK beta, I kappa B kinase beta, IkBKB, IKK 2, IKK B, IKK2, IKKB, Inhibitor of kappa light chain gene enhancer in B cells, Inhibitor of kappa light polypeptide gene enhancer in B cells, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta, Inhibitor of nuclear factor kappa B kinase beta subunit, Inhibitor of nuclear factor kappa B kinase subunit beta, MGC131801, NFKBIKB, Nuclear factor NF kappa B inhibitor kinase beta, Nuclear factor of kappa light chain gene enhancer in B cells inhibitor, IKKB_HUMAN.

Produktbeschreibung

Phospho-IKK beta (Tyr199) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 87 kDa
• UniProt: O14920
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: IKBKB

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Immunohistochemical staining of rat brain tissue using IKK beta (phospho-Tyr199) antibody.

Immunohistochemical staining of human placenta tissue using IKK beta (phospho-Tyr199) antibody.

Paraformaldehyde-fixed, paraffin embedded (mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (orb5519) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (orb5519) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (orb5519) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-IKK beta (Tyr199) (orb5519) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 85/87 kD.

Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-IKK beta (Tyr199) Polyclonal Antibody, Unconjugated (orb5519) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-IKK beta (Tyr199) Polyclonal Antibody, Unconjugated (orb5519) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.