FNIP1 Antibody: Biotin, Rabbit, Polyclonal

Produktübersicht

• Artikelname: FNIP1 Antibody: Biotin, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB55605
• Hersteller Artikelnummer: orb55605
• Alternativnummer: BYT-ORB55605-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the mid-protein of Human FNIP1
• Konjugation: Biotin
• Alternative Synonym: FNIP1 antibody, Folliculin-interacting protein 1 antibody, Folliculin interacting protein 1 antibody, KIAA1961 antibody

Produktbeschreibung

Rabbit polyclonal antibody against FNIP1 conjugated to Biotin.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: 130 kDa
• NCBI: 001008738
• UniProt: Q8TF40
• Puffer: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Target-Kategorie: FNIP1

Anwendungsbeschreibung

• Application Verdünnung: WB (1:1000), ICC/IF (1:100), IHC (1:50)
• Anwendungsbeschreibung: Application Notes: A 1:1000 dilution of SPC-718 was sufficient for detection of FNIP1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody. Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain, DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody. Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody. Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa.