Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB5565
• Hersteller Artikelnummer: orb5565
• Alternativnummer: BYT-ORB5565-100,BYT-ORB5565-200,BYT-ORB5565-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human, Rabbit, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105 IL(p-Y)RP
• Konjugation: Unconjugated
• Alternative Synonym: PKM2(phospho Tyr105), PKM2(phospho Y105), PK 1, PK 2, PK 3, PK Muscle type, PK1, PK2, Pk3, PKL, PKLR, PKM 2, PKM, PYKM, Pyruvate kinase 1, Pyruvate kinase 2/3, Pyruvate kinase 3, Pyruvate kinase isozyme R/L, Pyruvate kinase isozymes M1/M2, Pyruvate kinase liver and blood cell, Pyruvate kinase liver and RBC, Pyruvate kinase liver and RBC type, Pyruvate kinase M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, Pyruvate kinase type L, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK, TCB, THBP 1, THBP1, Thyroid hormone binding protein cytosolic, CTHBP, Cytosolic thyroid hormone binding protein, MGC3932, OIP 3, Oip3, Tumor M2-PK, p58, OIP-3.

Produktbeschreibung

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 58 kDa
• UniProt: P14618
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: PKM

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-PKM2 (Tyr105) antibody (orb5565), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

phosphopeptide, non phosphopeptide.

Tissue/Cell: human endometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PKM2 Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat Thyroid tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-NMDAR1 (Ser890) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugati Phospho-PKM2on to the secondary antibody and DAB staining.

Tissue/Cell: rat thyroid tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.