Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody, Clone: [1H12], Unconjugated

Produktübersicht

• Artikelname: Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody, Clone: [1H12], Unconjugated
• Artikelnummer: BYT-ORB559171
• Hersteller Artikelnummer: orb559171
• Alternativnummer: BYT-ORB559171-100,BYT-ORB559171-25,BYT-ORB559171-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human YAP1 around the phosphorylation site of S127 AH-pS-SP
• Konjugation: Unconjugated
• Alternative Synonym: phospho-YAP1(S127), YAP1(S127), YAP1(phospho Ser127), YAP1(phospho S127), 65 kDa Yes associated protein, YAp 1, YAP 65, YAP, YAP2, YAP65, Yes associated protein 1 65kDa, Yes associated protein 1, Yes associated protein 2, YKI, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1_HUMAN.

Produktbeschreibung

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1mg/ml
• Klon-Bezeichnung: [1H12]
• Molekulargewicht: 55 kDa
• UniProt: P46937
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: YAP1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-YAP1 (Ser127) antibody (orb559171), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Duodenum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Western blot:HepG2(Human) Cell,Primary:Anti-Phospho-YAP1 (Ser127) at 1:1000 dilution.