ACE2 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: ACE2 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB638863
• Hersteller Artikelnummer: orb638863
• Alternativnummer: BYT-ORB638863-50,BYT-ORB638863-100,BYT-ORB638863-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human ACE2 (351-450/805aa)
• Konjugation: Unconjugated
• Alternative Synonym: ACE-2, ACE 2, Angiotensin converting enzyme 2, ACE related carboxypeptidase, ACEH, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme(peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, DKFZP434A014, EC 3.4.17, angiotensin-converting enzyme 2 precursor, ACE2_HUMAN, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2.

Produktbeschreibung

ACE2 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 87 kDa
• UniProt: Q9BYF1
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: ACE2

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=2ug/Test

Bilder

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ACE2) polyclonal Antibody, Unconjugated (orb638863) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ACE2 antibody (orb638863), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10000 events was performed.

Sample: Lane 1: ACE2 knockout (KO) A549 Cell Lysate, Lane 2: Human A549 Cell (Control) Lysate, Primary: Anti-ACE2 (orb638863) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 120 kD.

Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ACE2 (orb638863) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Ovary (Mouse) Lysate at 40 ug, Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-ACE2 (orb638863) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Western blot:Lane 1:SH-SY5Y (Human) Cell,Lane 2:HepG2 (Human) Cell,Lane 3:293T (Hu