Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.
filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow, pH: 7.5 0.5
Formel:
C32H51N8O17P3S
Anwendungsbeschreibung:
Application Notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data, Nick Translation with DNAse I/DNA Polymerase I, Primer Extension with Klenow exo-, 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) , Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: lambdamax 294 nm, epsilon 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
Chemical structure of using Biotin-16-dCTP (Biotin) (Small Molecules)
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