Acetylated Lysine Antibody (Biotin), Rabbit, Polyclonal

Produktübersicht

• Artikelname: Acetylated Lysine Antibody (Biotin), Rabbit, Polyclonal
• Artikelnummer: BYT-ORB67532
• Hersteller Artikelnummer: orb67532
• Alternativnummer: BYT-ORB67532-400
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IF, IP, WB
• Spezies Reaktivität: All
• Immunogen: Acetylated KLH Conjugated
• Konjugation: Biotin
• Alternative Synonym: N6-Acetyl-L-lysine, N-epsilon-Acetyl-L-lysine, Nepsilon-Acetyllysine, N6-Acetyllysine, Acetyllysine

Produktbeschreibung

Rabbit polyclonal to Acetylated Lysine(Biotin). Detects proteins containing acetylated lysine residues in SDS PAGE immunoblots.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.25 mg/ml
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target-Kategorie: Acetylated Lysine

Anwendungsbeschreibung

• Application Verdünnung: WB (1:250), ICC/IF (1:100)
• Anwendungsbeschreibung: Application Notes: A 1/250 dilution of SPC-156 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis

Bilder

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000.