CD162 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: CD162 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB6814
• Hersteller Artikelnummer: orb6814
• Alternativnummer: BYT-ORB6814-100,BYT-ORB6814-200,BYT-ORB6814-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, WB
• Spezies Reaktivität: Human
• Immunogen: KLH conjugated synthetic peptide derived from human PSGL-1 (251-350/412aa)
• Konjugation: Unconjugated
• Alternative Synonym: CD 162, CD162, CD162 antigen, CLA, Cutaneous lymphocyte associated associated antigen, P selectin glycoprotein ligand 1, P selectin glycoprotein ligand 1 precursor, P-selectin glycoprotein ligand 1, PSGL 1, PSGL-1, PSGL1, Selectin P ligand, SELPL_HUMAN, SELPLG.

Produktbeschreibung

CD162 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 41 kDa
• UniProt: Q14242
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: SELPLG

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1µg /test

Bilder

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD162 antibody (orb6814), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb6814, 1 µg/1x10 6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CD162) polyclonal Antibody, Unconjugated (orb6814) 1:25, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Jurkat (Human) Cell Lysate at 30 ug, MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD162 (orb6814) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, U937 (Human) Cell Lysate at 30 ug, Primary: Anti-CD162 (orb6814) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.